Chromophore/fluorophore-assisted light inactivation method
نویسندگان
چکیده
منابع مشابه
Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans
Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory function. Chromophore-Assisted Light Inactivation (CALI) using genetically-encoded p...
متن کاملChromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis.
OBJECTIVES Expression of the nuclear Ki-67 protein (pKi-67) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi-67 is still missing. Rece...
متن کاملOptogenetic Inhibition of Synaptic Release with Chromophore-Assisted Light Inactivation (CALI)
Optogenetic techniques provide effective ways of manipulating the functions of selected neurons with light. In the current study, we engineered an optogenetic technique that directly inhibits neurotransmitter release. We used a genetically encoded singlet oxygen generator, miniSOG, to conduct chromophore assisted light inactivation (CALI) of synaptic proteins. Fusions of miniSOG to VAMP2 and sy...
متن کاملChromophore-assisted light inactivation and self-organization of microtubules and motors.
Chromophore-assisted light inactivation (CALI) offers the only method capable of modulating specific protein activities in localized regions and at particular times. Here, we generalize CALI so that it can be applied to a wider range of tasks. Specifically, we show that CALI can work with a genetically inserted epitope tag; we investigate the effectiveness of alternative dyes, especially fluore...
متن کاملSuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Chromophore-assisted light inactivation (CALI) is a powerful technique for acute perturbation of biomolecules in a spatio-temporally defined manner in living specimen with reactive oxygen species (ROS). Whereas a chemical photosensitizer including fluorescein must be added to specimens exogenously and cannot be restricted to particular cells or sub-cellular compartments, a genetically-encoded p...
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ژورنال
عنوان ژورنال: Folia Pharmacologica Japonica
سال: 2012
ISSN: 0015-5691,1347-8397
DOI: 10.1254/fpj.140.226